Job description
Position Details
Position Information
Recruitment/Posting Title
Postdoctoral Fellow
Job Category
Staff & Executive - Research (Laboratory/Non-Laboratory)
Department
NJMS-Microbiology
Overview
New Jersey’s academic health center, Rutgers Biomedical and Health Sciences (RBHS) takes an integrated approach to educating students, providing clinical care, and conducting research, all with the goal of improving human health. Aligned with Rutgers University–New Brunswick and collaborating university wide, RBHS includes eight schools, a behavioral health network, and six centers and institutes that focus on cancer treatment and research, neuroscience, clinical and translational research, advanced biotechnology and medicine, environmental and occupational health, and health care policy and aging research.
Our faculty are teachers, clinicians, and scientists with unparalleled experience who advance medical innovation and provide patient care informed by the latest research findings. We offer an outstanding education in medicine, dentistry, pharmacy, public health, nursing, biomedical research, and the full spectrum of allied health careers.
Our clinical and academic facilities are located throughout the state including New Brunswick, Piscataway, Newark, Scotch Plains, Somerset, Blackwood, and other locations. Clinical partners include Robert Wood Johnson University Hospital in New Brunswick, University Hospital in Newark, and other affiliates.
Through this community of healers, scientists, and scholars, Rutgers is equipped as never before to transform lives.
Rutgers RBHS is an anchor institution that boasts locations in Newark, New Jersey, a city of promise.
Posting Summary
Rutgers, The State University of New Jersey is seeking a Postdoctoral Fellow for the Department of Microbiology, Biochemistry and Molecular Genetics within the New Jersey Medical School. A postdoctoral fellow position is available in the Petrou lab at Rutgers-New Jersey Medical School (Newark, NJ). Our research focuses on the structural biology of membrane proteins using single particle cryo-electron microscopy (cryoEM). Our goal is to characterize the structure and function of bacterial membrane enzymes involved in antibiotic resistance, and eukaryotic receptors and enzymes relevant to human physiology and pathology.
The Petrou Lab is located in Newark, New Jersey, 20 min away from New York city. The Rutgers New Jersey CryoEM/ET facility, located in Piscataway, New Jersey, provides access to cryoEM and cryoET instrumentation including a 200kV Talos Arctica microscope, an Aquilos 2 FIB-SEM microscope, and a Leica cryo-CLEM setup. In addition, a local cryoEM facility at NJMS that will house a 100kV Tundra microscope equipped with speed-enhanced Ceta camera, and sample preparation equipment, will begin operating in the next few months, and is located in the same building as the Petrou Lab.
The Postdoctoral Fellow will be responsible for the expression, purification and structure determination by single-particle cryoEM of membrane proteins relevant to polymyxin antibiotic resistance. In addition, the Postdoctoral Fellow will be responsible for biophysical or biochemical characterization of target proteins, including protein-ligand or protein-protein interactions. Moreover, the Postdoctoral Fellow will assist the PI in lab organization, daily operations, personnel training, and grant writing activities.
The successful candidate will have a Ph.D. in Structural Biology, Molecular Biology, Biochemistry, or related fields. Preference will be given to candidates with experience in membrane protein expression and purification and/or single-particle cryoEM. Good communication & organizational skills and the ability to work independently are important for this position. Salary will be commensurate to the candidate’s experience and qualifications and based on the Rutgers postdoc salary schedule.
Interested candidates should e-mail a short cover letter detailing their background, CV and a list of three references to Dr. Vasileios Petrou (vasileios.petrou@rutgers.edu).
Among the key duties of this position are the following:
Among the key duties of this position are the following:
- Performs the following research-related tasks:
- a. Protein expression and purification – overexpression using bacterial, insect or mammalian systems, SDS-PAGE, and chromatographic separation using FPLC and HPLC.
b. Biochemical characterization of recombinant and native proteins (Western blotting, immunoprecipitation, cross-linking, etc.)
c. Molecular biology (expression vector design and construction, PCR, site-directed mutagenesis, in vitro transcription, etc.)
d. Biophysical characterization of recombinant or native proteins and their cognate targets (i.e. ligands or proteins) using Microscale thermophoresis (MST).
e. Sample preparation for single-particle cryoEM using cryo-plunging equipment (i.e. Vitrobot or equivalent).
f. Screening and data collection of single-particle samples using the Tundra or Arctica transmission electron microscopes.
g. Determination of three-dimensional structures of proteins using cryo-electron microscopy data and appropriate computational programs/resources (e.g. Relion, cryoSPARC, etc.). - Collects, compiles and presents data with appropriate conclusions, recommendations, etc. pertinent to research activity.
- Communicates regularly with the Principal Investigator.
- Maintains detailed and accurate experimental records.
- Participates and presents in journal clubs, seminars and conferences.
- Prepares reports for peer-reviewed scientific publications, and contributes to grant writing.
- Performs training of lab personnel in molecular biology, biochemistry and single-particle cryoEM.
- Supports the daily operations within the lab, e.g., lab reagent generation and administrative tasks.
- Performs laboratory inventory monitoring and ordering.
Position Status
Full Time
Hours Per Week
Daily Work Shift
Day
Work Arrangement
FLSA
Exempt
Grade
99S
Position Salary
$54,840-$57,852 depends years of experience
Annual Minimum Salary
Annual Mid Range Salary
Annual Maximum Salary
Standard Hours
37.50
Union Description
Non-Union
Payroll Designation
PeopleSoft
Benefits
Rutgers offers a comprehensive benefit program to eligible employees. For details, please go to http://uhr.rutgers.edu/benefits/benefits-overview.
Rutgers also offers a benefit program to eligible full-time postdoctoral fellow students. For details, please go to https://finance.rutgers.edu/healthcare-risk/insurance/postdoctoral-health-insurance.
Rutgers also offers a benefit program to eligible full-time postdoctoral fellow students. For details, please go to https://finance.rutgers.edu/healthcare-risk/insurance/postdoctoral-health-insurance.
Seniority Unit
Terms of Appointment
Temporary Staff Appointment - Salaried 12 Month
Position Pension Eligibility
None
Qualifications
Minimum Education and Experience
- Ph.D. in Structural Biology, Molecular Biology, Biochemistry, or related fields.
- Preference will be given to candidates with experience in membrane protein expression and purification and/or single-particle cryoEM.
- Good communication & organizational skills and the ability to work independently are important for this position.
Certifications/Licenses
Required Knowledge, Skills, and Abilities
Preferred Qualifications
Equipment Utilized
Physical Demands and Work Environment
PHYSICAL DEMANDS:
Must be able to safely perform laboratory experiments.
Must be able to safely perform laboratory experiments.
WORK ENVIRONMENT:
Research laboratory
Special Conditions
Posting Details
Posting Number
22ST3378
Posting Open Date
Special Instructions to Applicants
Position is contingent upon continued grant funding.
Regional Campus
Rutgers Biomedical and Health Sciences (RBHS)
Home Location Campus
Newark (RBHS)
City
Newark
State
NJ
Location Details
Pre-employment Screenings
All offers of employment are contingent upon successful completion of all pre-employment screenings.
Immunization Requirements
Under Policy 60.1.35 Immunization Policy for Rutgers Employees and Prospective Employees, Rutgers University requires all prospective employees to provide proof that they are fully vaccinated and have received a booster (where eligible) against COVID-19 prior to commencement of employment, unless the University has granted the individual a medical or religious exemption. Employees who are not eligible for a booster at the time of an offer of employment must provide proof they have received a booster upon eligibility and upload proof of same. Under Policy 100.3.1 Immunization Policy for Covered Individuals, if employment will commence during Flu Season, Rutgers University may require certain prospective employees to provide proof that they are vaccinated against Seasonal Influenza for the current Flu Season, unless the University has granted the individual a medical or religious exemption. Additional infection control and safety policies may apply. Prospective employees should speak with their hiring manager to determine which policies apply to the role or position for which they are applying. Failure to provide proof of vaccination for any required vaccines or obtain a medical or religious exemption from the University will result in rescission of a candidate’s offer of employment or disciplinary action up to and including termination.
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